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JANUS HENDERSON FUND MANAGEMENT UK LIMITED THE CITY OF LONDON INVESTMENT TRUST PLC LEGAL ENTITY IDENTIFIER: 213800F3NOTF47H6AO55 1 September 2023 THE CITY OF LONDON INVESTMENT TRUST PLC Total Voting Rights In conformity with DTR 5.6.1 we would like to notify the market of the following information. As at 31 August 2023, The City of London Investment Trust plc's issued share capital consisted of: a) 502,664,868 Ordinary Shares of 25p; b) ?301,982 of First Preference Stock; c) ?507,202 of Second Preference Stock; and d) ?589,672 of Preferred Ordinary Stock. The number of voting rights in The City of London Investment Trust plc is: a) 33,510,991 in respect of the Ordinary Shares (one vote for every 15 shares held); b) 30,198 in respect of First Preference Stock (one vote for every ?10 of stock held); c) the Second Preference Stock does not have voting rights; and d) 29,484 in respect of Preferred Ordinary Stock (one vote for every ?20 of stock held). Therefore, the total number of voting rights in The City of London Investment Trust plc as at 31 August 2023 was 33,570,673. The above figure (33,570,673) may be used by shareholders as the denominator for the calculations by which they will determine if they are required to notify their interest in, or a change to their interest in, The City of London Investment Trust plc under the FCA's Disclosure Guidance and Transparency Rules. For further information, please call: Sally Porter For and behalf of Janus Henderson Secretarial Services UK Limited Corporate Secretary to The City of London Investment Trust plc Telephone: 020 7818 2550 Harriet Hall PR Manager, Investment Trusts Janus Henderson Investors Telephone: 020 7818 2919 End CA:00417560 For:TCL Type:MKTUPDTE Time:2023-09-04 08:30:03